Device
Part:BBa_K771008:Design
Designed by: Guo Huaqing Group: iGEM12_SJTU-BioX-Shanghai (2012-09-25)
Membrane Anchor 5 without VVD:ssDsbA-LGT-GBD Ligand
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504
Design Notes
For both Membrane Accelerator and Membrane Switch
Source
Lgt and ssDsbA are from E.coli genome; GBD ligand obtained from Prof. John Dueber